RISE offers in vitro analysis to study interactions between the small intestine and substances or microbiota.

We offer the in vitro modell EpiIntestinal to study the small intestine:

EpiIntestinal (MatTek Corporations) is a in vitro model based on human primary cells from the small intestinel The model simulates a human small intestine which one can use to study uptake rate, uptake mechanisms, inflammatory responses, gene expression, cytokine production, toxcicity and interactions with microbiota and more. When studying bioavailability of potential drug substances or effect of probiotica this is a prefered model to choose to be able to screen many candidates fast to be able to choose the best ones earlier in the development phase. The model is more translatable to human situation and correlates better with human clinical absorption data than the older Caco2 model. We are happy to assist you when you deside upon parameters, study design and read outs. Everything can be adjusted according to your needs. 

By screening substances in vitro before going in vivo you will save both money and research animals by finding the best candidates in an earlier part of the development. By choosing the EpiIntestinal model could help translating the in vitro results into real utake in humans, since data from rodents not always are translatable to humans. It is also posible to investigate interactions with microbiota specific for humans. 

When ordered, the coosen model system are put into cultivation for that specific customer project making it important to have all aspects of the study design in place before cultivation starts. When the models are ready, 4 weeks later, they are usually exposed with the test substances for two hours. Samples are then taken from either the supernatant or the cells depending on parameters of interest for the customer. If the study aim to detect anti inflammatory effect in teh small intestine we can both look at cytokine secretion or gene expeession. To ensure that the membrane integrity is high, permeability is tested on each replicate with lucifer yellow as a marker or with TEER. This information will be included in the report together with the parameters of interest for the customer.

The customer is provided with a report including values for parameters of interest for their substances and quality assurance of the membranes. 

1,5 month after accepted tender. Since cultivation of the modelsystems takes 4 weeks we recommend you to discuss study design with one of the contact persons before putting an order ensuring that the right amount of replicatates are used. Exposure time also affects delivery time. Weekends or national holidays will affect the delivery time. 

Information regarding test subtstance need to be discussed before start of cultivation of the model systems. The test substances need to arrive one week befor test start.

RISE offer an in vitro model to study pro, anti and inflammatory attributes of subtances in the signal path of NF-kB.

Inflammatory responses in humens are mainly associated with the NF-kB signaling pathway. This response is triggered by substances from bacteria and virus but also by other types of stress like UV light or chemical substances. When a substance trigger a NF-kB response an inflamation is initiated in the body.

Sometimes when we are sick and have a high activation of NF-kB we want to ease the symptoms related to inflammation (eg. fever) by taking anti inflammatory pharmaceuticals (eg acetyl slicylic acid or paracetamol).

To introduce novel substances or food sources in our diet or local environment it is important to know if these can cause an infammation in persons exposed to them or if they can have the posibility to lower the inflammatory response.

We offer an simple model giving the posibility to study many samples at the same time in an in vitro model. This model can determine if a substance is pro, anti or neutral regarding an inflammatory respons in the NF-kB signaling pathway. 

By evaluating substances in vitro before going in vivo, both reseach animals and money can be saved by choosing the best candidate in an earlier stage of the development.

The model is based on GloResponse™ NF-κB-RE-luc2P HEK293. This is a transformed cell line expressing an enzyme (luciferace) under the NF-kB-promotor. When the NF-kB gene is activated, the enzyme will be expressed. One measures the activation by adding substrate (luciferin) for the enzyme and the end point is luminiscence.

If one is interested in inhibition of this signal patwhay one stimulates the cells with TNF-a to get an enhanced background level before trying to lower this activation with substances of choise. To eliminate false positive, were the effect seen is due to an toxic response leading to cell death instead of lowering readout, this version of the method is coupled with an toxcicity assay as controlls. 

The customer will get a report containing parameters of choice and qulity assurance data of the toxcicity of the substances.

It ususally takes two weeks to start up cells from cryopreservation. More over time for the specific test will add on making the total time of 4 weeks from acceptance of tender. Contact us for a more specific time frame.

Weekends or national holidays will affect the delivery time. 

Information regarding test subtstance need to be discussed before start of cultivation of the model systems. The test substances need to arrive one week before test start.

RISE offers an in vitro method to study the uptake of substances that are to be oraly administred.

We offer two different modells to study uptake in the small intestine:

- Caco2 is the classical in viitro method used to study uptake in small intestine. The modell uses cancer cells originating in the colon. These cells will spontaneously differentiate to someting similar to the small intestine. The membrane integrity are still similar to that of the colon.

- EpiIntestinal is a mor modern method using human primary cells originating from the small intestin. This modell correlate better with human clinical data than Caco2.

We will help you choose the modell fit for your experiments. Study design can be adjusted to fit your whishes regarding concentrations, controlls and exposure time. 

Evaluating substances in vitro befor moving into in vivo will save both research animals and money by eliminate unfit candidates at an earlier time in the development. Testing on EpiIntestinal might also help translate your data to expected uptake in human since the animals usually tested for uptake befor human is rodents. EpiIntestinal correlates better with the human clinical data than the rodents in some aspects.

When ordered, the coosen model system are put into cultivation for that specific customer project making it important to have all aspects of the study design in place before cultivation starts. When the models are ready, 4 weeks later, they are usually exposed with the test substances for two hours. Samples are then taken from  the original solution, lumen and blod side if the models and presented to the customers as Papp values. 

If the substance arent to be found in the lumen or blood side of the modell we will suggest adding on a analysis of the membrane to see if it is associated to the cells or the membrane or if it has been metabolized. 

To ensure that the membrane integrity is high, permeability is tested on each replicate with lucifer yellow as a marker or with TEER. This information will be included in the report together with the permeability of the substances (Papp values).

The customer will get a report including permeability (Papp) for the specific substances and quality controll of the membranes. 

1,5 month after accepted tender. Since cultivation of the modelsystems takes 4 weeks we recommend you to discuss study design with one of the contact persons before putting an order ensuring that the right amount of replicatates are used. Exposure time also affects delivery time. Weekends or national holidays will affect the delivery time. 

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Information regarding test subtstance need to be discussed before start of cultivation of the model systems. The test substances need to arrive one week before test start.