Using molecular biological analysis methods, we can investigate how different molecules and systems within the cell interact and how these processes are regulated. We can examine differences within the cell system at genome and protein level as well as verify the authenticity of a sample.
One can examine how a product is affecting the biological function by looking at how different molecules and systems within the cell interact.
Quantitative PCR (qPCR) is a method that copies DNA using primers (short DNA fragments). Primers are adapted to the gene/DNA of interest. Primers are complementary to the DNA and are bound by a polymerase. The polymerase is copying the DNA to millions of copies that is trackable and quantifiable using a fluorophore.
At times when the expression of a particular gene is of interest, RNA converted to DNA is analysed.
Report with (depending on the methods chosen):
- RNA/DNA concentration and quality
- Revers transcription and amplification program
- Cq values
- Amplifications curves
- Genex analis on demand
Molecular biological analyses at genetic level
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