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Determination of antiviral activity of textile products

Name of service (page headline, shown in promos – maximum of 70 characters incl. spaces): ISO 18184 antiviral activity of textile products Lead (include SEO-words and the main benefits for your target groups. Stick to one paragraph, maximum 2-3 sentences):

This standard determines the antiviral activity of the textile product against specific viruses.

Purpose/Benefit:

This standard includes testing methods to determine the antiviral activity of a textile product. The tests use specified viruses but due to individual sensitivities, the results gathered from one virus cannot be directly transferred to another virus. However, any textile product can be tested including woven fabrics, knitted fabrics, yarns, braids, or individual fibers.

Method (what/which methods are used to perform the service):

The viruses are deposited onto a specimen. After a specific contact time, the remaining infectious virus is counted, and the reduction rate is calculated by the comparison between the antiviral product test specimen and a control specimen.

The standard test is performed using influenza virus and Feline calicivirus. However, it can also be done with model organisms like bacteriophages or other types of virus on request.

Delivery (what does the client get after performed service – e.g. a report, certificate etc.):

The test results are compiled in technical reports. Depending on the purpose of the test, summaries or preliminary results may be obtained on request.

Area:
Infection control
Life Science
Medical devices
Textile
Contact person (Enter one name per field. Activated personal contact pages will appear automatically): Sarunas Petronis, Forskare
Textil
Field measurements: No Price type: 1 Division: Division Bioeconomy Preparation: No preparation required Standards:

ISO 18184

Certification and marking: Not applicable Type of service: Testing / Analysis / Evaluation Instrument: Not applicable General area: Not applicable Order information: Kontakta någon av personerna nedan för beställning Divison (OLD): Division Bioeconomy Delivery level: Not applicable
lucia.gonzales.stromberg@ri.se
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3. Good health and well-being
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Textiles Sekundär områdes navigation:
Metrology
Infection control
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Clothing for protection against contact with blood and body fluids

Name of service (page headline, shown in promos – maximum of 70 characters incl. spaces): ISO 16604, Clothing for protection against pathogens Lead (include SEO-words and the main benefits for your target groups. Stick to one paragraph, maximum 2-3 sentences):

ISO 16604, Clothing for protection against contact with blood and body fluids

Purpose/Benefit:

The method determines the resistance of protective clothing materials to penetration by blood-born pathogens using bacteriophage ΦX174 as a model organism.

Method (what/which methods are used to perform the service):

A specimen is subjected to a nutrient broth containing a virus in a test apparatus for a specific period of time and pressure sequence. Visual detection of penetration is done by detecting viable viruses which penetrate the material.

Delivery (what does the client get after performed service – e.g. a report, certificate etc.):

The test results and conclusions are presented in a written report. 

Area:
Infection control
Life Science
Medical devices
Textile
Contact person (Enter one name per field. Activated personal contact pages will appear automatically): Sarunas Petronis, Forskare
Virus
Field measurements: No Price type: 1 Division: Division Bioeconomy Preparation: No preparation required Standards:

ISO 16604

Certification and marking: Not applicable Type of service: Testing / Analysis / Evaluation Instrument: Not applicable General area: Not applicable Order information: Contact one of the persons below to order or for further information Divison (OLD): Division Bioeconomy Delivery level: Not applicable
lucia.gonzales.stromberg@ri.se
/en/node/9710
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Infection control Sekundär områdes navigation:
Physical protection and safety
Metrology
Textiles
Tjänstetyp tagg: Provning

Viral Filtration Efficiency (VFE)

Name of service (page headline, shown in promos – maximum of 70 characters incl. spaces): Viral Filtration Efficiency (VFE) Lead (include SEO-words and the main benefits for your target groups. Stick to one paragraph, maximum 2-3 sentences):

The VFE test determines how well a given material filters virus-containing droplets of about 3 microns in size.

Purpose/Benefit:

The “Viral filtration efficiency” (VFE) test is a modification of the SS-EN 14683 standard, which uses the bacteriophage ΦX174 as the challenge organism.

Method (what/which methods are used to perform the service):

A material with filtration properties is clamped between a six-stage cascade impactor and an aerosol chamber. An aerosol of bacteriophage ΦX174 is introduced into the aerosol chamber and drawn through the filter material and the impactor under vacuum. The VFE of the tested material is given by the number of plaque forming units passing through the material expressed as a percentage of the number of plaque forming units present in the challenge aerosol. A higher VFE means that the material has higher effectivity to function as a barrier against viruses.      

The test is done in a BSL-2 laboratory and can be performed with other types of bacteriophages on request.

Delivery (what does the client get after performed service – e.g. a report, certificate etc.):

The test results and conclusions are presented in a written report. 

Area:
Infection control
Life Science
Textile
Contact person (Enter one name per field. Activated personal contact pages will appear automatically): Charlotta Löfström, Senior Forskare Field measurements: No Price type: 1 Division: Division Bioeconomy Preparation: No preparation required Certification and marking: Not applicable Type of service: Testing / Analysis / Evaluation Instrument: Not applicable General area: Not applicable Order information: Contact one of the persons below to order or for further information Divison (OLD): Division Bioeconomy Delivery level: Not applicable
lucia.gonzales.stromberg@ri.se
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Viruses in microbiological safety

Viruses in microbiological safety

We offer analysis methods and expertise in a broad range of areas that include viruses.

RISE offers services and knowledge about infectious disease viruses to meet the challenges and needs of the society and the industry.

Background

The Covid-19 pandemic has highlighted the importance of studying viruses in different contexts alongside the clinical work. This has opened up the opportunity to raise virus-related issues within the industry and the society.

Focus

  • Pandemic preparedness, prevention and resilience.
  • Detection.
  • Analysis of Inactivation/Killing
  • Evaluation of the effectiveness of products (hygiene products & food), water & air, substances (antiviral drugs), chemicals (biocides), coating etc.
  • Advice & risk assessment regarding the manufacturing process.

Services and Methods

Analysis of filters

  • Virus filtration efficiency (VFE)
  • Virus recovery from filters

Virucidal effects on surfaces

  • Filters
  • Textiles
  • Soft surfaces
  • Hard surfaces

Efficiency analysis

  • ISO-methods
  • Customize methods

Detection and typing

  • qPCR
  • Sequencing 

Model organisms

  • ISO-methods
  • Eukaryote virus 
  • Bacteriophages

Risk assessment

  • Process, products and materials
  • Theoretical and practical

Charlotta Löfström

Senior Forskare
+46 10 516 67 30 Read more about Charlotta
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Division (OLD): Division Bioeconomy Division: Division Bioeconomy Infection control

Assessment of material potential to induce antimicrobial resistance

Induction of antimicrobial resistance
Pseudomonas aeruginosa

A sustainable product development includes the assessment of risks to induce or select for antimicrobial resistance. RISE has a broad competence in evaluating risks for resistance development using microbiology analysis methods combined with in-depth-knowledge of cellular processes.

Exposure of bacteria to antimicrobial substances or materials can lead to the evolution of resistance towards the active agent or an increased abundance of resistant bacteria through resistance selection, even at low antimicrobial concentrations. Development of antimicrobial resistance is a global threat to society and therefore it needs to be strongly considered when choosing or developing new antimicrobial technologies.

A sustainable development of antimicrobial substances or surfaces includes the assessment of risks to induce or select for antimicrobial resistance. RISE has a broad expertise in microbiological analysis combined with in-depth-knowledge of cellular and environmental processes that lead to increased antimicrobial resistance. The development of antimicrobial resistance in microbes can be tested and quantified in various ways depending on the material and antimicrobial properties.

The following methods can be applied and individually customized to evaluate the potential to induce or select for antimicrobial resistance:

  • Antimicrobial susceptibility testing before and after short- or long-term exposure
  • Multistep resistance assay to test if resistance can be induced
  • Identification of the induced resistance mechanism through:
    • Whole genome sequencing to detect genomic changes
    • Transcriptomic analysis through qPCR (relevant for e.g. drug efflux pumps, cellular stress response)
    • Phenotypic alterations (microbial growth analysis, yield etc.)
  • Test for cross-resistance through a comprehensive resistance screen including e.g. antibiotics, biocides, metals
  • Competition experiments between susceptible and resistant strains to analyze the risk for selection of antimicrobial resistance
  • Application of advanced cultivation systems (biofilm reactors, growth in suspension)
  • Support in field experiments; characterization of the microflora
  • Measuring the antimicrobial activity against common antibiotic resistant pathogens (e.g. MRSA or carbapenem resistant Klebsiella aeruginosa)

Nadine Kraupner

Forskare
+46 10 516 55 35 Read more about Nadine

Contact Nadine

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Division (OLD): Division Materials and Industry Division: Division Materials and Industry Infection control

Choice of material in case of risk of contact infection.

Name of service (page headline, shown in promos – maximum of 70 characters incl. spaces): Choice of material in case of risk of contact infection. Lead (include SEO-words and the main benefits for your target groups. Stick to one paragraph, maximum 2-3 sentences):

In healthcare, there is an increased risk of spreading infection. Infection can be transmitted through touch surfaces such shared between patients. This imposes special requirements when cleaning and disinfecting. RISE has the competence to evaluate the suitability of the choice of materials and chemicals intended for the healthcare environment.

Purpose/Benefit:

Requirements for materials:

In the healthcare sector, all materials that are present must be easy to clean and durable. This places great demands on the nature of the materials as cleaning, with both mechanical and chemical action, is carried out regularly in these environments.  It can be difficult to determine whether a material such as in a bathtub, chair covering or mattress can withstand to be cleaned and disinfected repeatedly over a long period of time  while the removal of dirt and microorganisms is still sufficient.

Disinfectant requirements:

Surfaces with patient contact where infection could be transmitted must be cleaned and sometimes disinfected. Special requirements are imposed on the disinfectant in terms of killing microorganisms while the chemicals must not damage the material. RISE can evaluate the microbiological effect and assess what the material can withstand.

Method (what/which methods are used to perform the service):

With a broad expertise in materials and surfaces, chemistry and analytical methods, microbiological requirements and methodology, RISE offers the following expertise and services related to hygiene, cleaning and disinfection of surfaces in healthcare environments:

  • Effectiveness of disinfectants against bacteria, yeast, mold and viruses
  • Biofilm, detection, identification, build-up, reduction
  • Bioburden, detection on surfaces
  • Cleanability test, worst case scenario with relevant dirt
  • Material compatibility with chemical disinfectants
  • Surface resistance to abrasive wear
  • Accelerated aging and simulated exposure to environmental factors

Tests are, as far as possible, equal to or based on relevant ISO and EN standards.

Delivery (what does the client get after performed service – e.g. a report, certificate etc.):

An assignment at RISE regarding material selection can, for example, constitute support in product development work, procurement or purchasing in order to be able to choose between different solutions to obtain a suitable, i.e. durable and easy-to-clean material. It can also involve evaluating the microbiological or dirt-removing effect of a disinfectant or cleaning agent.

Area:
Infection control
Chemical and biological analysis
Medical devices
Product safety
Contact person (Enter one name per field. Activated personal contact pages will appear automatically):
Sarunas Petronis, Forskare
Charlotta Löfström, Senior Forskare
Surfaces in hospital care.
Field measurements: No Price type: 1 Division: Division Materials and Industry Preparation: No preparation required Certification and marking: Not applicable Type of service: Not applicable Instrument: Not applicable General area: Not applicable Order information: Please, contact the person(s) listed below Divison (OLD):
Division Bioeconomy
Division Materials and Industry
Delivery level: Not applicable
sarunas.petronis@ri.se,charlotta.lofstrom@ri.se
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Biofilm on surfaces, filters and in pipes can, among other things, give rise to production stop and production defects.

Name of service (page headline, shown in promos – maximum of 70 characters incl. spaces): Biofilm on surfaces - a problem in many industries Lead (include SEO-words and the main benefits for your target groups. Stick to one paragraph, maximum 2-3 sentences):

Biofilm formation is the microorganisms' way of protecting themselves against factors in the environment such as chemicals, antibiotics, heat etc. Biofilm therefore creates problems in many industries and environments, for example in water management in various contexts, in healthcare, in the paper industry and not least in the food industry.

Purpose/Benefit:

Research has shown that when a biofilm has formed, the microorganisms can be up to a thousand times less sensitive to, for example, antibiotics than when they live freely in a solution. Correspondingly, resistance to cleaning and disinfection chemicals, biocides, heat, but also to drying out increases.

The microorganisms that can form biofilm can in some cases cause disease if they are found on surfaces in the food industry ex. Listeria, in healthcare ex. Enterococci or in shower water in the form of Legionella. In the paper and other process industries, the biofilm can manifest itself, for example, in the form of product defects or clogged filters.

RISE can, with various methods, help prevent biofilm formation, for example by choosing the right type of material, means or design of equipment. We can also offer risk assessment in situations where risks may arise due to biofilm formation e.g. with stagnant water and the possibility of growth and spread of Legionella. 

 

Method (what/which methods are used to perform the service):

We have methodology for testing and evaluation at different scales, from lab scale to large-scale in the form of smaller reactors and larger rigs, where you can circulate water to simulate biofilm construction and removal of biofilm. Combinations of different materials, chemicals, cleaning regimes and biofilm types can be evaluated.

At our microbiological lab, which is BSL 2 classified, we can analyze biofilm with traditional culture techniques, flow cytometry and MBEC to look at levels of microorganisms as well as with molecular techniques to get an identification of the microorganisms that occur.

 

Delivery (what does the client get after performed service – e.g. a report, certificate etc.):

Examples of deliveries to customers: 

  • Basis for being able to choose suitable materials that counteract biofilm construction
  • Help and advice to be able to design in a way that makes it difficult to build biofilms
  • Evaluation of the effectiveness of chemicals in counteracting and removing biofilm
  • Risk assessment and preventive measures with a focus on Legionella in water systems

 

Area:
Infection control
Chemical processes and products
Chemical and biological analysis
Life Science
Food
Pulp and paper
Production and manufacturing
Product safety
Contact person (Enter one name per field. Activated personal contact pages will appear automatically): Charlotta Löfström, Senior Forskare Field measurements: Yes Price type: 1 Division: Division Bioeconomy Preparation: No preparation required Certification and marking: Not applicable Type of service: Innovation services Instrument: Not applicable General area: Not applicable Order information: Kontakta ansvariga personer Divison (OLD): Division Bioeconomy Delivery level: Not applicable
charlotta.lofstrom@ri.se
/en/node/9710
3. Good health and well-being
6. Clean water and sanitation
12. Responsible consumption and production
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Trouble shooting microbiological deviations in production processes

Name of service (page headline, shown in promos – maximum of 70 characters incl. spaces): Trouble shooting microbiological deviations Lead (include SEO-words and the main benefits for your target groups. Stick to one paragraph, maximum 2-3 sentences):

Sometimes things goes wrong in the production and the products are microbiologically spoiled or even worse pathogens are detected in the products. Then it is important to know how to handle the situation to establish why it occurred, what to do about it and how to prevent it happening again. RISE microbiologists can help you trouble shooting.

Purpose/Benefit:

Trouble shooting normally starts with identifying the nature of the microbiological problem to get a first clue to where it might  stem from. This also gives an input to in what environment the organism can survive and grow. Sometimes an extensive sampling all along the production line is perfomed including raw materials and product in different stages, as before and after an inactivation step as for example a heat process.

Method (what/which methods are used to perform the service):

Depending on the cause of the problem some  or all of the steps below might be used in the trouble shooting process.

The work begins with a joint visit to the facility by a microbiologist and a process technician. The visit is made because it is of the utmost importance to understand the problem on the spot in order to understand it.

Samples might be collected, to find the microorganism causing the issue, not only from the raw mateial and produt in different phases but from all factory environment as open surfaces with product contact, in packaging area, air, rinsing water after CIP-process and more . 

Measuring temperatures  and effective time in any inactivation/heat process inside the actual product might also be needed. Based on the data calculations to determine if the inactivation/heat process is enough to kill the microorganism in focus, will be done.

Routines and observance for cleaning and disinfection are also often looked into.

Review of protocoll from microbiological analysis over some time might also be included to understand how the problem have increased over time.

Delivery (what does the client get after performed service – e.g. a report, certificate etc.):

When the cause of the problem has been found measures are taken to prevent it happening again. Corrective actions are suggested  and monitoring and control are looked over. All work are summarised in a written report.

Area:
Control
Life Science
Food
Water
Contact person (Enter one name per field. Activated personal contact pages will appear automatically):
Charlotta Löfström, Senior Forskare
Lars Hamberg, Senior Forskare
Production equipment on site.
Field measurements: Yes Price type: 1 Division: Division Bioeconomy Preparation: No preparation required Certification and marking: Not applicable Type of service: Innovation services Instrument: Not applicable General area: Not applicable Order information: Contact person Divison (OLD): Division Bioeconomy Delivery level: Not applicable
charlotta.lofstrom@ri.se,lars.hamberg@ri.se
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More secure laundry using mathematical models

Washroom

When the covid-19 pandemic struck in the spring of 2020, Electrolux Professional realized that their customers, including those outside the hospital and healthcare segment, had an increased need for laundry and drying products that were guaranteed to disinfect textiles. However, testing the effectiveness of products on actual bacteria and viruses is very costly. Therefore, researchers at RISE, based on several scientific studies on inactivation of bacteria and viruses, have created mathematical models to predict the disinfection capacity of laundry and drying products.

Electrolux Professional, which this year celebrates 120 years, offers complete solutions with washing machines, dryers, mangles and other equipment for professional use. In the company's concept, it is important to be able to guarantee customers functional products and that it is possible to determine the degree of microbiological killing obtained in each step of the washing and drying process.

– Before the pandemic, it was mainly customers in the Care segment, ie hospitals and healthcare, who demanded a washing process where viruses and bacteria are deactivated and killed, says Silvana Johansson, Global Head of Category Laundry at Electrolux Professional. When the pandemic struck in the spring of 2020, people became anxious due to the risk of infection, and we quickly realized that even those of our customers who take care of, for example, hotel laundry and laundry rooms must be able to offer guaranteed hygienically clean laundry.

Correct time and temperature

In order to achieve an optimal process and end result, it is a matter of combining washing and drying times with the right temperatures.

– In an efficient, safe and sustainable washing and drying process, bacteria are killed and viruses are inactivated - something that of course came into extra focus during the pandemic. In this project, our task has been to help Electrolux Professional in their development work, where one aspect has been to increase knowledge about which parameters play a role in efficiency and to test different products. By using mathematical models, we can make a microbiological risk assessment in a cost-effective way, says Erik Nygren, project manager at RISE and researcher in process technology, safety and hygiene.

It is important for us to be able to offer a washing process based on research and facts

Required to work even with incorrect dosage

In large washing plants, dosing of detergent usually takes place automatically, while in smaller plants dosing is done manually. Therefore, it has been an important aspect of the development work that the killing and deactivating effect on bacteria and viruses should be independent of the human factor – even if the detergent were incorrectly dosed, the combination of the program's temperature and time will guarantee a hygienically clean wash.

In order to be able to study deactivation and killing of viruses and bacteria present in a product or in a production plant, practical experiments have often been used in the past with contaminated washcloths that passed through the washing machine, which were then analyzed after washing. The advantage of working with mathematical modeling is that you take advantage of the great knowledge and established methodology that is already used to study at what temperatures different microorganisms die or are inactivated. Using mathematical models in this way is a classic methodology from the food and process industry area. RISE has, among other things, done similar assignments where deactivation of microorganisms in recycled paper fibers from paper napkins were studied.

– Some viruses are always relevant for the customer to work with, such as winter vomiting. What RISE offers is an understanding of which viruses and bacteria we really need to deactivate and relate to, what is required to kill or deactivate, the amount of microorganisms in the process, as well as the consequences of different amounts and types of microorganisms, says Erik Nygren, who worked in the project together with colleague Lars Hamberg, researcher in process technology, safety and hygiene at RISE. For example, a vomiting disease is not such a serious infection for most people, but the perceived feeling is very stressful.

Combination of competences

By combining two different core competencies, RISE has contributed with an understanding of the evaluation of various infectious diseases, process technical understanding and engineering thinking. This is to be able to map processes together with the customer and mathematically model them in order to get a figure on how much viruses and bacteria are deactivated and killed in the washing process.

– The challenge lies in understanding the customer's actual needs to be able to meet these. Another important part is the educational aspect, as it is part of RISE's overall goal to strengthen the competitiveness of Swedish industry, says Erik Nygren. We must not only deliver a product, but also an understanding - for example of how to identify and prevent the spread of disease-causing or product-destroying microorganisms. In this project, we model on laundry and drying products, but it can just as easily be about recycling food packaging.

Erik Nygren believes that demand for this type of service will increase, especially from customers who need to work with microbiological risk assessments based on the Swedish Work Environment Authority's regulations. RISE is well equipped to take on similar assignments as for Electrolux Professional in several sectors, both through strong interdisciplinary expertise, where customers do not have to turn to several players to solve the task, and through a well-proven methodology combined with a large database of current microorganisms.

– We have worked together with RISE in various contexts for many years, so it was an obvious contact to make when we realized the seriousness of the new situation that the pandemic entailed, says Silvana Johansson. It is important for us to be able to offer a washing process based on research and facts, as that is our guarantee that our customers get a safe product. We have benefited greatly from the collaboration and received a very good delivery in the form of project management, shared knowledge, reports and webinars.

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Microbiological Purity of Products in Food Contact

Name of service (page headline, shown in promos – maximum of 70 characters incl. spaces): Microbiological Purity of Products in Food Contact Lead (include SEO-words and the main benefits for your target groups. Stick to one paragraph, maximum 2-3 sentences):

Microorganisms in liquids, paper, and cardboard can cause staining and contamination, impacting both the production process and the final product. In food-related manufacturing, it is particularly important to monitor and control this to ensure high standards of quality and hygiene.

Purpose/Benefit:

In the manufacturing of paper and cardboard intended for food contact, it is crucial to carefully monitor the bacterial content of the final product. High bacterial levels can not only impact food safety but also cause issues in the production process. Bacteria can contribute to operational disruptions by forming biofilms in pipelines and on machine components, leading to production downtime and increased maintenance costs. Moreover, microbial growth can result in discoloration or odors in the finished material, which in turn may lead to customer complaints and quality deviations.

To control bacterial levels, standardized testing methods are used to provide an indication of the microbial load in the paper or packaging. While these methods do not always give a complete picture of the specific microorganisms present, they serve as essential tools for ensuring compliance with hygiene standards. Regular monitoring and proper maintenance of production equipment are also crucial measures to minimize the risk of bacterial contamination and to ensure that paper and cardboard products are safe for food contact.

Method (what/which methods are used to perform the service):

The method ISO8784-1:2014 do not say everything, we know e.g. not which ones grow, but the method is standardized and works well as a control to use even in mills.

Delivery (what does the client get after performed service – e.g. a report, certificate etc.):

RISE offers a report that includes data in Excel format and relevant images and diagrams. It is carefully compiled using standard methods for the conducted analyses. 

Delivery time:

The delivery of results is scheduled based on our order backlog and the scope as well as the complexity of your assignment. We aim to ensure that your results are delivered on time, taking into account the specific requirements of your project.

Area:
Bioeconomy
Biorefinery
Packaging
Chemical and biological analysis
Life Science
Food
Pulp and paper
Testing
Contact person (Enter one name per field. Activated personal contact pages will appear automatically): Christina Wedin, Coordinator
Bacteria
Field measurements: Yes Price type: 1 Division: Division Bioeconomy Preparation: No preparation required Standards:

RISE offers services for sampling and mapping of processes to assess and monitor various parameters critical to production efficiency and product quality. 

Certification and marking: Not applicable Type of service: Testing / Analysis / Evaluation Instrument: Not applicable General area: Not applicable Order information: Beställning via kontaktperson Divison (OLD): Division Bioeconomy Delivery level: Not applicable
christina.wedin@ri.se,
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