Jump directly to content

Spinnability evaluation of new polymer materials

Name of service (page headline, shown in promos – maximum of 70 characters incl. spaces): Spinnability evaluation of new polymer materials Lead (include SEO-words and the main benefits for your target groups. Stick to one paragraph, maximum 2-3 sentences):

RISE in Mölndal conducts research and development of textile fibres, with a special focus on material transition for a sustainable development. Melt spinning poses certain requirements on the material, including high temperature stability and suitable flow properties. We can evaluate the spinnability of newly developed materials.

Purpose/Benefit:

Melt spinning is the most commonly used industrial fibre spinning method. It’s great advantage is that the method requires only heat, no solvents, to transform polymers into a melt which can be extruded and drawn into thin fibres.

Globally, there is at present a rapid development of thermoplastics from biobased and recycled feedstocks, and consequently melt spinning will be increasingly useful to produce environmentally sustainable textile fibres.

The fibre spinning expertise RISE can support material producers by performing evaluation of the spinnability of new polymer materials. This evaluation requires only small amounts of polymer (approx. 100 grams) and is suitable also for R&D materials.

If the material is found to have good spinnability, larger scale trials and pilot fibre production can be carried out in our equipments for lab-scale and/or semi-industrial fibre spinning.

Method (what/which methods are used to perform the service):

Several material properties are relevant for spinnability, and our evaluation includes characterisation of:

  • Degradation temperature, using thermogravimetric analysis (TGA)
  • Melting point and degree of crystallinity, measured by differential scanning calorimetry (DSC)
  • Composition analysis, for example by FTIR
  • Flow properties (shear viscosity and extensional viscosity) as a function of shear rate and temperature, as measured in a capillary rheometer (Netzsch RH10)
  • Melt strength as a function of take-up rate, measured in a capillary rheometer
  • Small scale extrusion of monofilaments, in capillary rheometer
  • Mechanical characterisation of fibres, by tensile testing
Delivery (what does the client get after performed service – e.g. a report, certificate etc.):

Small amounts of fibres on a bobbin.
Report in Swedish or English.

Delivery time:

According to agreement.

Area: Material transition Contact person (Enter one name per field. Activated personal contact pages will appear automatically):
Sabrina Kopf, Forskare
Ezgi Ceren Boz Noyan, Forskare
Melt spun textile fibres
Field measurements: No Price type: 1 Division: Division Materials and Industry Preparation: Description of preparation Preparation information:

According to agreement.

Certification and marking: Not applicable Type of service: Innovation services Instrument: Not applicable General area: Not applicable Order information: For more information or ordering, please contact the person(s) listed below. URL: /en/materials-and-durability/textiles/test-demo/textile-fiber-development-test-… Divison (OLD): Division Materials and Industry Delivery level: Not applicable
sabrina.kopf@ri.se,ezgi.ceren.boz.noyan@ri.se
Description for CTA: Läs mer om vår testbädd för textilfiberutveckling
/en/about-rise/operations/mission-governance/policy-documents/privacy-policy
Documents:
9. Industry, innovation and infrastructure
12. Responsible consumption and production
Order
form
Textiles Sekundär områdes navigation:
Metrology
Production and manufacturing
Biotechnology
Chemical products and processes
Tjänstetyp tagg: Provning

NextGenProteins

NextGenProteins
NextGenProteins

På den globala marknaden växer efterfrågan på högkvalitativt protein för både mat och foder. På grund av den växande världens befolkning, resursbrist och klimatförändringar blir efterfrågan på protein svårare att tillgodose.

Partner
Completed
Bioraffinaderi
Region Västernorrland
2023-09-30
7 985 150 €
Division: Division Bioekonomi

På grund av denna höga efterfrågan är en av vår tids mer angelägna frågor att säkerställa en kontinuerlig och hållbar tillgång på proteiner för både människor och djur. Alternativa proteinförsörjningskedjor bör utvecklas i enlighet med de vetenskapliga principerna för cirkulär ekonomi och livscykelbedömning.

Projektet NextGenProteins fokuserar på uppskalning och optimering av alternativ proteinproduktion. Under projektet bedöms lämpligheten av tre olika typer av alternativa proteiner (mikroalger, insekter och svampar (SCP)) som högkvalitativa proteiningredienser i foder och livsmedel. Man utforskar hur man kan inkludera dessa i specifika, riktade foder- och livsmedelstillämpningar och hur de kan optimeras för att möta kundernas behov och säkerställa konsumenternas acceptans. 

Projektet kommer att bidra till att stärka livsmedelsförsörjningen, hållbarheten och självförsörjningen av EU:s proteinproduktion genom att visa lämpligheten och den ekonomiska livskraften för nästa generations proteiner som en del av värdekedjorna för livsmedel och foder.

RISE roll i projektet 

På RISE Processum fokuserar man på uppskalning och optimering av svampproteinproduktion (kallad SCP: single cell protein) från underutnyttjade skogsbiomassarester samt på nedströmsprocesserna för att möta industriella och regulatoriska krav för fodertillämpningar. RISE Processum är även arbetspaketledare för produktionsprocesserna och utvärdering och optimering av funktionella och näringsmässiga egenskaper i projektet.

Charilaos Xiros

Senior researcher, co-ordinator of Research Biotech Group
+46 10 516 67 84 Read more about Charilaos
Profile image

Contact Charilaos

CAPTCHA

* Mandatory 

By submitting the form, RISE will process your personal data.
2.Ingen hunger
9.Hållbar industri, innovationer och infrastruktur
12.Hållbar konsumtion och produktion
13.Bekämpa klimatförändringarna
15.Ekosystem och biologisk mångfald
MOWI CIRCULAR
Projekt logo: Logotype Project end date: Livsmedel Sekundär områdes navigation:
Cirkulär omställning
Bioteknik
Biobaserade cirkulära processer

Biobaserade energibärares bidrag till ett flexibelt energisystem

Bioflex

I skiftet mot fossilfrihet är elektrifiering ett viktigt verktyg, men elektrifieringen medför också ett kraftigt ökat elbehov. Förnyelsebar energi från sol- och vindkraft behöver balanseras för att skapa redundans i energisystemet. I kombination med andra förnyelsebara energibärare, så som biogas och vätgas, kan redundansen i energisystemet utökas.

Koordinator
Completed
Cirkulär omställning Energi Fossilfria drivmedel Klimatneutral industri Vatten Vätgas
Region Skåne
3 år
4 853 183 SEK
Division: Använd ej - Division Samhällsbyggnad

Den industriella omställningen har stött på hinder och utmaningar i att kunna säkra tillräckligt med förnyelsebar energi, oberoende av energikälla eller energibärare (t.ex. el, biogas, vätgas). Effektbristen och -problematiken i södra Sverige har fått exemplifiera några av de stora utmaningar som Sveriges energisystem står inför, både regionalt och nationellt. Effektbristen har bland annat medfört regionala problem så som stoppad utbyggnation av existerande industrier och nyetablering av andra.

Bioflex syfte var att öka flexibiliteten, redundansen och robustheten i energisystemet genom att integrera biologiskt producerade energibärare (biovätgas och biogas) med elektrolytisk vätgas. Projektet undersökte möjliga synergieffekter mellan energibärarnas olika produktionsvägar, kombinerad anaerob rening (rötning) och biovätgasproduktion samt elektrolytisk vätgas, och mellan energibärarna i sig.

Kombinationen av energibärare och produktionsvägar förväntandes generera tre huvudsakliga effekter: effektivare resursutnyttjande, ökad andel biobaserad energi och ökad flexibilitet i lokala energisystem. Ökad resurseffektivitet uppnås då bioprocesserna tar vara på restströmmar ex. industriellt avloppsvatten för produktion av biogas och biovätgas, men även då resurser delas mellan bioprocessen och elektrolysen.

Projektet genomförde labb- och pilotstudie av en tvåstegs-bioprocess, för kombinerad produktion av biogas och biovätgas, undersökte synergieffekter och flexibilitet i samspelet mellan bioprocesserna och elektrolytisk vätgas, teknoekonomisk analys av systemet och en analys av implementeringsscenarion. Projektmålen var att demonstrera tvåstegs-bioprocessen kontinuerligt, minska uppehållstiden i biogasproduktionen och att skapa en implementeringsplan och rekommendationer till aktörer för fortsatt utveckling av konceptet.

Erika Lönntoft

Projektledare
Read more about Erika
Profile image

Contact Erika

CAPTCHA

* Mandatory 

By submitting the form, RISE will process your personal data.
6.Rent vatten och sanitet
7.Hållbar energi för alla
9.Hållbar industri, innovationer och infrastruktur
11.Hållbara städer och samhällen
12.Hållbar konsumtion och produktion
Rapporter från Bioflex
Attach document:

Slutrapport (pdf, 1.19 MB)



Project end date: Vätgas Sekundär områdes navigation:
Cirkulär omställning
Bioteknik

BIOMAC

BIOMAC
BIOMAC

In March 2020, the European Commission adopted a new circular economy action plan aimed at preparing the European economy for a green future and strengthening its competitiveness while protecting the environment.

Partner
Completed
Biorefinery
Region Västernorrland
2024-12-31
16 596 702 €
Division: Division Bioeconomy

One of the keys to a successful implementation of the European circular economy is the growing dependence on biomass products. A wider use of such products can solve several problems associated with the disposal of solid waste, as they could be reused at the end of their life cycle, ensuring full circularity. BIOMAC will fill an existing gap regarding nanostructured bio-based materials (NBM). NBM can be the solution to several of the challenges that society faces in achieving a circular economy. In the current situation, there are major shortcomings in terms of investments, financing, upscaling and further development for NBM, which makes the material difficult to obtain commercially available.

BIOMAC will provide services to stakeholders and work to accelerate the entry of NBM into the market. To achieve this, BIOMAC OITB will develop tailor-made solutions for producing and integrating nanomaterials throughout the bio-based value chain. BIOMAC will at the same time offer services that cover assessment of regulation and safety, sustainability and circularity. The project establishes a concrete community of open collaboration for stakeholders and customers that enables innovation and minimizes investment risks.

In the project, RISE Processum will use fiber rejects, a residual stream from the forest industry, as a raw material for the production of bacterial nanocellulose (BNC). Fermentable sugar is extracted from the fiber repellent and used by the bacteria to produce BNC. The nanocellulose will then be used by other project partners in application tests for improved properties in packaging materials and smart textiles. In parallel, RISE Processum is collaborating with the project partners to expand the use of fermentable sugars from fiber sludge for the production of more nanomaterials useful in various applications.

Goals

The ultimate goal of BIOMAC is to help address barriers that prevent the development of concept development in the field of nano-activated bio-based materials and products, providing a unique opportunity to test ideas in a well-structured open environment and to industrialize a new generation of nano-activated bio-based materials. The ambition of the project is to strengthen and maintain innovation in the field of European bioeconomic industries, by reducing the time to market for new nanotechnologies, and thus reducing costs and risks.

Charilaos Xiros

Senior researcher, co-ordinator of Research Biotech Group
+46 10 516 67 84 Read more about Charilaos
Profile image

Contact Charilaos

CAPTCHA

* Mandatory 

By submitting the form, RISE will process your personal data.
9. Industry, innovation and infrastructure
12. Responsible consumption and production
13. Climate action
17. Partnerships for the goals
BE ECO ISQ Group
Projekt logo: BIOMAC logotype Project end date: Biobased materials Sekundär områdes navigation:
Circular transition
Packaging
Biotechnology
Production and manufacturing

Like:meat

Like:meat
Like:meat

The interest in plant-based meat-like products, so-called meat analogues, is large and growing. In this project, RISE, together with Lantmännen and Orkla, aimed to develop useful and attractive meat analogues based on Swedish raw materials.

Coordinator
Completed
Food
Not applicable
2 år
4 500 000 SEK
Division: Division Bioeconomy

Final seminar 25th of October 2023 (online) >>

 

Aim

The project aimed to develop next-generation Swedish plant-based meat analogues, which are more nutritious and attractive than today's alternatives. The products will be the first to be based entirely on Swedish raw materials and the first to be completely processed and produced in Sweden.

Challanges

For today's plant-based meat analogues, there are a number of nutritional challenges. The bioavailability of iron and zinc is likely to be low, due to the presence of antinutritional factors, such as phytic acid. Oligosaccharides in legumes can cause abdominal pain and flatulence. The composition of amino acids is also limited to current processable proteins (soy, pea and gluten).

Some legumes used in today's meat analogues are grown in Sweden, but the protein extraction has so far not been carried out in Sweden, nor has the extrusion of the products.

Solution

In this project extrusion was used is combination with fermentation. Through fermentation, the nutritional quality can increase by reducing the content of antinutritional factors (e.g. phytic acid). The content of oligosaccharides can also be expected to decrease. Fermentation can also facilitate successful extrusion, by improving protein quality and reducing the starch content of protein concentrates. Another positive effect of fermentation may be a reduced off-flavour of extruded legume protein.

The project had an interdisciplinary approach and takes place in collaboration between industry and institute, which contributed expertise in food and materials science, food processing, nutrition, fermentation, simulation / modeling and product development.

Mats Stading

Senior forskare
+46 76 127 26 03 Read more about Mats
Profile image

Contact Mats

CAPTCHA

* Mandatory 

By submitting the form, RISE will process your personal data.

Johanna Eckardt

Forskare
+46 70 319 67 37 Read more about Johanna
Profile image

Contact Johanna

CAPTCHA

* Mandatory 

By submitting the form, RISE will process your personal data.
3. Good health and well-being
12. Responsible consumption and production
Lantmännen R&D Orkla Foods Sverige
Project end date: Food Sekundär områdes navigation:
Circular transition
Biotechnology
Agriculture

Viscose Process - Spinning Pilot

Viscose Process - Spinning Pilot
Spinning pilot dissolving

Increased population in the world results in increased consumption of textiles. This means that the textile raw material needs to be optimised and its process developed to make them more sustainable so that the consumption of the earth's resources is reduced. In that context, the spin pilot is excellent for new techniques and ideas about textils.

Laboratory testbeds (LT)
Region Västernorrland

Nadine Hollinger

Forsknings- och utvecklingsingenjör
+46 10 722 32 22 Read more about Nadine

Contact Nadine

CAPTCHA

* Mandatory 

By submitting the form, RISE will process your personal data.

Diana Reyes Forsberg

Forskare
+46 10 722 33 88 Read more about Diana
Profile image

Contact Diana

CAPTCHA

* Mandatory 

By submitting the form, RISE will process your personal data.
Off
Division: Division Bioeconomy

Overview of the spinning process 

Viscose solution is pressed through small holes into a spinning bath which can contain various chemicals such as sulfuric acid, zinc sulphate or sodium sulphate. Carbon disulfide disappears, and cellulose precipitates. The precipitate is drawn into a continuous thread. 

Offer in viscose pilot

In the spin pilot, we can use different compositions of the spin bath and thus offer customers projects throughout the chain from raw material based on lignocellulose to finished thread. You can test an existing process or a future process. 

By evaluating both the spinning process and the properties of the thread, knowledge about the dissolving cellulose for further processing into technical or textile viscose materials can be gathered. RISE has a complete chain of pilots; from chipping, boiling and bleaching via viscose solution to finished viscose thread in the spin pilot. 

It is common for pulp manufacturers to investigate their possibilities of increasing their dissolving production or, from a completely different segment, to convert the process towards this area. In evaluating the pulp quality, the viscose fibre is characterised. 

Materials Process industry Pulp, paper and packaging
Bioeconomy Biorefinery Circular transition Pulp and paper Textile
BioInnovation
Division (OLD): Division Bioeconomy Biobased materials Sekundär områdes navigation:
Circular transition
Biotechnology

DNA sequencing of microorganisms and microbiota

Name of service (page headline, shown in promos – maximum of 70 characters incl. spaces): DNA sequencing of microorganisms and microbiota Lead (include SEO-words and the main benefits for your target groups. Stick to one paragraph, maximum 2-3 sentences):

Using DNA sequencing and bioinformatics, microorganisms and microbiota can be identified and characterized with high precision in products, processes, clinical and biological samples, as well as in the environment. We support all stages of your sequencing project—from experimental design to translating complex sequencing data into decision-making.

Purpose/Benefit:

DNA is the foundation of most microbial biology, making sequencing a powerful tool with many applications. It can be used to trace microbial contamination in production facilities, analyze microbiota composition in clinical samples, identify infection biomarkers and vaccine candidates, or detect resistance genes in isolated bacteria. Beyond harmful microbes, we also work with beneficial microorganisms in products, processes, microbiota, or the environment (e.g., food production microbes or bacteria that degrade persistent compounds). For example, we can monitor microbiota changes during fermentation or product storage.

A key advantage of sequencing is the ability to analyze the entire microbiome—not just cultivable organisms.

How RISE can support your sequencing project?

We conduct sequencing projects involving viruses, bacteria, yeasts, and molds, as well as antimicrobial resistance and other traits. Our expertise spans sampling and DNA extraction from complex samples, with deep understanding of customer needs. Our knowledge is built through in-house research and close collaboration with industry in applied and contract research.

We can also complement genomics or metagenomics with other omics approaches (e.g., transcriptomics, proteomics) to gain deeper insights. Multi-omics enables solving complex problems previously out of reach, especially when integrated using advanced data analysis and AI.

We offer full or partial project support, including:

  • Study design and planning: methodology, sampling strategy, sequencing technique selection, and analysis planning.
  • Sampling from complex environments, products, and biological materials.
  • Sample preparation and DNA extraction in Bio Safety Level 2 (BSL2) labs.
  • Sequencing using short-read (e.g., Illumina) and long-read (e.g., Oxford Nanopore) technologies:
    • Whole genome sequencing of bacterial isolates for identification, outbreak tracing, resistance gene detection, virulence markers, novel functions, etc.
    • Amplicon sequencing (e.g., 16S rRNA, ITS) for microbiota composition analysis.
    • Shotgun metagenomics for characterizing unknown microorganisms and microbiota.
  • Bioinformatics analysis (e.g., strain comparison, SNP analysis, MLST, cgMLST, genome assembly from metagenomes) for product development, quality control, benchmarking, outbreak tracing, microbiome profiling, biomarker discovery, etc.
  • AI-driven integration of genomics/metagenomics with other omics data for interpretation and decision support.
  • Customized training
Method (what/which methods are used to perform the service):

We use modern high-throughput sequencing technologies, including Next-Generation Sequencing (NGS, e.g., Illumina) and third-generation sequencing (e.g., Oxford Nanopore). Depending on project needs, we apply and adapt microbiological methods from sampling and DNA extraction to sequencing and data interpretation.

We can also integrate other omics methods such as transcriptomics, proteomics, metabolomics, glycomics, and lipidomics.

Project overview DNA sequencing

  1. Sample collection
    Representative samples are collected from products, processes, clinical materials, or environments. Microorganisms are isolated or microbial DNA is extracted, purified, and quality-checked.
  2. Library preparation and sequencing
    Extracted DNA is prepared for sequencing and processed in sequencing instruments that read the DNA sequence.
  3. Bioinformatics
    Powerful computing tools filter and analyze the large volumes of sequencing data. Genomes are reconstructed and compared to reference databases (e.g., ENA, GenBank) to identify organisms and genes of interest (e.g., resistance, virulence).
  4. Analysis and interpretation
    Our research team interprets the findings, studying genetic variants and their implications using databases, literature, and custom scripts tailored to the project.
  5. Results
    A report is produced summarizing the sequencing findings and analyses. If needed, we also provide oral presentations to support decision-making.
Delivery (what does the client get after performed service – e.g. a report, certificate etc.):

The results are summarized in a report and presented orally to the client.

Area:
Bioeconomy
Infection control
Agriculture
Chemical and biological analysis
Life Science
Food
Pulp and paper
Product safety
Risk and safety
Water
Contact person (Enter one name per field. Activated personal contact pages will appear automatically):
Jonas Ravn, Forskare
Francisco Salva Serra, Forskare
DNA
Field measurements: No Price type: 1 Division: Division Bioeconomy Preparation: No preparation required Certification and marking: Not applicable Type of service: Testing / Analysis / Evaluation Instrument: Not applicable General area: Not applicable Order information: Beställ genom att ringa eller skicka e-post till kontaktpersonen Divison (OLD): Division Bioeconomy Delivery level: Not applicable
jonas.ravn@ri.se,francisco.salva.serra@ri.se
/en/about-rise/operations/mission-governance/policy-documents/privacy-policy
2. Zero hunger
3. Good health and well-being
6. Clean water and sanitation
9. Industry, innovation and infrastructure
12. Responsible consumption and production
Purpose - Header: Purpose Metod - Header: Methodology Delivery - Header: Deliverables
Order
form
Biotechnology Sekundär områdes navigation:
Water
Infection control
Food
Tjänstetyp tagg: Provning

Whole genome sequencing for studies of viruses in the hand microbiota

Sequencing of viruses in hand microbiota
Hands with microorganisms

The interaction between different viruses and microorganisms on the skin is complex as the microbiota is built up and maintained. Hygiene products should be designed so that pathogenic microorganisms are removed without affecting the microbiota. PCR and sequencing were used to map viruses and microorganisms on hands, surfaces and soft materials.

Project manager
Completed
Infection control Chemical and biological analysis Life Science
2016-2018
Division: Division Bioeconomy

Background

It has long been known that the human body is colonized by a diversified microbiota consisting of bacteria, archaea and fungi. It has only recently been noticed that viruses are also included in this microbiota, which is very important for our health. Published studies also show that certain physiological effects previously attributed to colonizing bacteria instead stem directly from replicating viruses.

All in all, there seems to be a complex interplay between different viruses and the microorganisms on our skin in the construction and maintenance of our microbiota, which in turn plays a crucial role in both good health and disease. Therefore, hygiene products for professional and private use should be designed so that pathogenic viruses, bacteria and fungi are eliminated while the impact on the composition of the microbiota is limited.

Challenge

The use of quantitative PCR and large-scale sequencing over the past ten years has revolutionized our understanding of both individual pathogenic bacteria and the communities of microorganisms in which they are included. The degree of innovation in this area has been high and the knowledge and technology that has been developed is now used successfully in both the pharmaceutical and food industries. In comparison with bacteriology, the development of our understanding of viruses and how they interact with our body has been more modest during the same time period. However, as a result of a number of major investments and recent technical innovations, the development and degree of innovation in virology is forecast to increase sharply in the coming years.

Objectives

The primary objective of the project was to develop and adapt PCR technology and whole genome sequencing for mapping and quantification of viruses and microorganisms on hands, surfaces and soft materials. In addition, the total microbiota on the hands was mapped and quantified before and after application of two different hand wash / disinfection methods on subjects.

Charlotta Löfström

Senior Forskare
+46 10 516 67 30 Read more about Charlotta
Profile image

Contact Charlotta

CAPTCHA

* Mandatory 

By submitting the form, RISE will process your personal data.
3. Good health and well-being
9. Industry, innovation and infrastructure
12. Responsible consumption and production
Project end date: Offer-pages: Research and innovation against antibiotic resistance, in line with the One Health approach Infection control Sekundär områdes navigation:
Biotechnology
Food
Chemical and biological analysis

Resistance genes in the hand microbiome

Resistance genes in the hand microbiome
Hands with microorganisms

Knowledge of the presence of resistance genes to antibiotics and biocides in microorganisms on the skin is important in order to be able to develop effective and long-term sustainable hygiene products. In the project, methodology for whole genome sequencing suitable for mapping resistance genes was further developed and applied.

Projektledare
Completed
Infection control Chemical and biological analysis Life Science
2017-2020
Division: Division Bioeconomy

Background

There are major socio-economic gains to be made from reducing the spread of disease-causing viruses and microorganisms in the home and public environment. In addition, reducing the spread of infection is a strategic priority area in the WHO's work to limit unnecessary antibiotic use and the development of resistance in society. Effective hygiene products and disinfectants for professional and private use are crucial in this work.

In order to be long-term effective against the spread of infection in society, it is of great importance that repeated use of these products does not harm the natural microbiota present on our skin, as the skin's normal microbiota is an important protection against infections. These products should also not cause resistance selection, as resistance to disinfectants may also give rise to increased resistance to antibiotics.

Challenge

Given the extensive societal challenges we face globally linked to increased problems with antibiotic and biocide resistance, there is today a great need for broader knowledge regarding the development of resistance. As a result of the rapid development that is now taking place in microbiology and virology in terms of tools for whole-genome sequencing and bioinformatics, there are today great opportunities to build new crucial knowledge and actively work to reduce the spread of resistance in society.

Methods

The project further develops and adapts a methodology for whole genome sequencing that is suitable for mapping resistance genes to antibiotics and biocides in microorganisms. This methodology was adapted for studies of the microflora on both hands, surfaces and in complex biological samples. In a study consisting of twelve subjects, the presence of resistance genes in the normal flora of the hands was mapped and quantified before, during and after a long-term use of long-acting disinfectants. 

Culture-based methodology was established and applied for studies of links between biocide resistance and antibiotic resistance, so-called cross-resistance. Specifically, in vitro studies were performed on how skin bacteria's resistance to the biocide benzalkonium chloride can arise, and how the cross-resistance to certain antibiotics then increases. To evaluate the societal relevance of the induced resistance, the most resistant bacteria were completely sequenced and the DNA code was compared with resistance genes identified in hospital samples.

Charlotta Löfström

Senior Forskare
+46 10 516 67 30 Read more about Charlotta
Profile image

Contact Charlotta

CAPTCHA

* Mandatory 

By submitting the form, RISE will process your personal data.
3. Good health and well-being
9. Industry, innovation and infrastructure
12. Responsible consumption and production
Project end date: Offer-pages: Research and innovation against antibiotic resistance, in line with the One Health approach Infection control Sekundär områdes navigation:
Data Science
Biotechnology
Chemical and biological analysis

Proposal for additional national standard hygienisation methods

More standard hygienisation methods
The corona virus

Enhanced economy at biogas plants and in fertilizer management with alternative national standard hygienisation
methods

Coordinator, participant
Completed
Bioeconomy Fossil free fuels Infection control Agriculture Product safety Risk and safety
3 years
2,8 MSEK
Division: Division Bioeconomy
Image: Daniel Tamm

To be able to merchandise manure or digestate, the material must be sanitized to ensure infection control. Swedish biogas facilities today have the choice between using one of two standard methods (at least 1 h at 70 °C, or 10 h at 52 °C) or going through a costly validation process if using alternative parameters. The main aim of this project has been to increase the profitability in the handling of biofertilizers by developing additional approved national standard methods for hygienization and thereby simplify the validation process. To achieve this, we have conducted interviews with stakeholders to find out which hygienisation parameters are most interesting for them. We have also compared the situation in other European countries and quantitatively and qualitatively reviewed scientific studies considering hygiene and inactivation data of microorganisms. However, the largest part of the project was laboratory studies where we generated novel inactivation data for the heat-tolerant bacterium Salmonella Senftenberg W775 at different temperatures and time intervals. The requirement for an approved hygienisation is a 5-log reduction of the bacteria. The choice of input parameters in the lab studies was based on literature as well as on the interviews where often preference for hygienization at lower temperature was expressed to save energy and to avoid the need to adapt the equipment to higher temperatures.

Another request that emerged from the interviews was that, in connection with emptying vessels, they want to avoid leaving bottom sediment for destruction, which is a costly procedure. Therefore, we have investigated the inactivation data of the roundworm Ascaris suum using limestome combined with heat treatment.

Based on our results, we propose the establishment of the following methods as new Swedish national methods for hygienisation:

  1. Heat treatment of substrate for 3.5 hours at 60 °C
  2. Heat treatment of substrate for 1 hour at 65 °C
  3. Heat treatment of pure, source-separated food waste for 1 hour at 52 °C
  4. Addition of at least 5 mass-% of lime (CaO) to bottom sediment, and ensuring a temperature of at least 65 °C for at least 15 minutes.

Our assessment and recommendation are that the Swedish regulating authority can use the data regarding proposed treatment methods to approve new or existing biogas plants without the need to carry out a complete validation process including in-situ killing trials.

Daniel Tamm

Forsknings- och utvecklingsingenjör
+46 10 516 69 75 Read more about Daniel
Profile image

Contact Daniel

CAPTCHA

* Mandatory 

By submitting the form, RISE will process your personal data.
Final report
Attach document: Project end date: Biotechnology Sekundär områdes navigation:
Circular transition
Water
Energy and electrification
Agriculture