Separation of chemical compounds

Purpose

HPLC can analyze more polar and thermally unstable compounds than gas chromatography (GC) and is therefore often a complement to this analysis technique.

Organic acids: formic acid, acetic acid, levulinic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid, hexanoic acid, heptanoic acid, methanol, ethanol, glycerol and lactic acid and the furan derivatives hydroxymethylfurfural (HMF) and furfural.

Examples of sample types where organic acids can be analyzed:

• Cooking liquids
• Acid liquor
• Yeast tank samples
• Black liquor

Optical brighteners are expensive chemicals that should preferably stick to the paper surface and end up in the drain as little as possible. Environmental reasons also speak for the latter. With HPLC analyzes, both qualitative and quantitative determinations of the presence of optical brighteners can be made in both paper and in different flows around the paper machine. In competing analyzes, it is often possible to determine whether the optical brightener used is the optical brightener of the di-, tetra- or hexate type.

Method

HPLC systems have the ability to control parameters such as eluent mixtures, gradients and temperatures, which makes it possible to analyze complex sample matrices. The system has a refractive index detector (RI) and an ultraviolet detector (UV) of the type Diode Array Detector (DAD), which means that you can get a complete UV-VIS spectrum of each compound.

In the same system, high-molecular material can also be analyzed and one can get a picture of the molecular weight distribution for e.g. lignin, lignosulfonate and other polymers. In the biorefinery of the future, there is a great need to analyze new associations, e.g. various components from lignin.

Deliveries

Report