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Light Microscopy Quickly Reveals Small details

With light microscopy, you can quickly gain an overview of the problem, and all images are displayed in color. It is often used in conjunction with product development, troubleshooting, and customer support. To gain understanding of a product, a microscopic image often guides you towards decisions regarding further approaches.

Purpose

In the field of fiber analysis and related studies, various types of assignments encompass a wide range of subjects and processes. Here's a more detailed explanation of the different types of assignments:

Fiber composition: Analysis of the composition of fibers in a sample, which may include identifying different types of fibers and their proportions in the material.

Fiber identification: Determining and classifying different types of fibers present in a sample, which can be important for understanding the material's properties and applications.

Fiber damage: Investigation of any damages to the fibers, which may have occurred during the manufacturing process or handling of the material.

Fiber studies: Exploration and analysis of various properties of fibers, such as their structure, size, and characteristics.

Vascular cells: Studies of vascular cells, which are a type of cells found in plant tissues and can affect the material's properties and quality.

Cross-sectional studies in the z-direction: Analysis of the cross-section of the material in the z-direction, which involves studying the material's layering and distribution of different substances and contaminants throughout its thickness.

Distribution of starch, OBA, printing ink in the z-direction: Examination of how substances like starch (a polysaccharide), optical brightening agents (OBA), and printing ink are distributed throughout the material's thickness.

Depositions, e.g., localization, tape pull-off: Studies of deposits on the material, such as identifying their localization or conducting investigations after tape pull-off.

In addition to these fiber-related assignments, other subjects and processes may also be included:

1. Magnesium hydroxide, MgOH2
2. Calcium carbonate, CaCO3
3. Bacteria
4. Coating
5. Printing ink
6. Bark
7. Microorganisms
8. Size measurements (e.g., fiber wall thickness, surface layer, coating thickness): Measurements of various dimensions and thicknesses on materials and coatings.

Additional areas include:

Surfaces and print quality: Studies of surfaces and print quality, which may include examinations of fibers on the surface, fiber protrusion (upright fibers), visible contaminants through the coating layer, defects in laminated surfaces, printing surfaces, and folds (creasing).

These assignments are conducted to understand and improve various aspects of material handling, manufacturing, and the quality of the final product across different industries and applications.

Method

Technical Information about Light Microscopy:

Light microscopy is a crucial tool for studying materials and biological samples at a microscopic level. Here's a more detailed explanation of the various technical features and capabilities available with a light microscope:

1. Transmitted Illumination:
  - Bright Field (BF): This is the most common form of illumination in light microscopy. Light passes through the examined object and is then reflected up into the eyepiece or captured by a camera to create an image.
  - Dark Field (DF): A special illumination method where light is refracted by the samples, making them visible against a dark background. This is useful for highlighting details in the sample that may otherwise be difficult to see in bright field.
  - Polarized Light: By using polarized light, one can analyze the optical properties and structure of materials. It is particularly useful for studying crystal structures and other materials that polarize light.
  - DIC (Differential Interference Contrast): An advanced technique that provides a three-dimensional image of the object by exploiting the interference of light rays passing through the sample. It provides high-contrast images of the object's details and structures.
  - Phase Contrast: Another method to enhance contrast in light microscopy, especially useful for studying living cells or other transparent objects without the need for staining.

2. Incident Illumination:
  - Bright Field (BF): Similar application as in transmitted illumination, but in this case, the light is directed towards the object from the side.
  - Dark Field (DF): Similar to transmitted illumination, but the light is reflected by the object and appears against a dark background.
  - Polarized Light: Can also be used in this illumination method to study the optical properties and structure of materials.
  - DIC (Differential Interference Contrast): Similar to transmitted illumination, DIC can be used to provide high-contrast images of the object's details and structures.

3. UV Light (Fluorescence Microscopy) at Various Wavelengths:
  - This feature is used to examine fluorescent materials in a sample, which can be useful for visualizing specific substances or structures labeled with fluorescent dyes or markers.

In addition to these features, other microscopy models may also be available, such as stereo microscopes for studying objects in three dimensions and microtomes for cutting thin sections of materials for further analysis.

Light microscopes can have a magnification limit of up to 1000 times and a resolution limit of approximately 0.2 micrometers, enabling detailed observations of microscopic structures and phenomena.

Expertise:

With our expertise in microscopy and extensive experience in pulp technology, we are well-equipped to perform a range of analyses and studies related to pulp, paper, cardboard, and other related materials. We always strive to deliver high-quality results and customized solutions that meet our clients' specific needs and contribute to their success in the market.

Deliveries

We tailor our result delivery according to your order, taking into account its scope and any complexity involved. This way, we can ensure that you receive your results on time and that they meet your expectations.

RISE offers a report that includes data in Excel format along with relevant images and diagrams. It is meticulously compiled using standard methods for the conducted analyses. The report also includes an assessment of measurement uncertainty to ensure the reliability of the presented results. All relevant parameters pertinent to the specific analysis are included to provide a comprehensive picture of the situation. Additionally, detailed comments and explanations are provided to offer further insight and understanding of the presented data.

RISE strives to ensure the highest possible quality and reliability in the presented results, which in turn assists customers and stakeholders in making informed decisions based on the provided information.

When it comes to the samples you send to us for analysis, your peace of mind is our priority. Unless otherwise agreed, we retain the samples for a period of four weeks after the analysis has been conducted. This way, we can ensure that you have ample time to review the results and make any further inquiries or actions if necessary. We always aim to provide the best service possible.

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Service

Light Microscopy Quickly Reveals Small details

Price

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Delivery time

The delivery of results is scheduled based on our order backlog and the scope as well as the complexity of your assignment. We aim to ensure that your results are delivered on time, taking into account the specific requirements of your project. Thank you for your understanding and patience as we work to provide you with the best possible service.

Preparations

During sampling, we would like to provide you with some friendly recommendations to ensure the best possible results:

1. Scraping into the container during sampling: Please scrape the sample into the provided container to ensure proper handling and analysis.

2. Avoid marking too close to the defect/problem: When marking the sample, make sure not to mark too close to the defect or problem area. This helps us obtain a clear and representative image of the sample.

3. Freezing is performed at RISE: We take care of the freezing of the sample when it arrives with us. This way, you can feel confident that we handle your sample in the best possible manner.

4. Provide references: If possible, please include references to process and functional chemicals/materials. This helps us better understand the context and enables us to conduct a more detailed analysis. It's also helpful if you have good reference samples to compare with problem or poor samples.

Thank you for taking the time to follow these recommendations. We are here to assist and ensure that your sampling and analysis process proceeds as smoothly and efficiently as possible.

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Jessica Sjöstedt

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Jessica Sjöstedt

Forsknings- och utvecklingsingenjör

+46 10 722 32 38

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