Skip to main content
RISE logo
DNA
Photo: Pixabay

New DNA-based methods for the skin microbiome

We will develop and validate robust DNA-based methods (qPCR and NGS) to quantify bacteria in the skin's microbiome. The project provides the basis for studies of the skin's microbiome to study, for example, the effect on the composition when applying different skin care products.

It has long been known that the human body is colonized by a diversified microbiome consisting of bacteria, archaea and fungi. In addition, it is well documented that imbalances between naturally occurring microorganisms caused by, among other things, antibiotic treatment have a crucial role in several skin diseases.

In order to study the composition and change of the microbiome, there is a need for sensitive and robust methods of analysis. The use of quantitative PCR (qPCR) and large-scale sequencing (NGS) has revolutionized our understanding of the composition and richness of skin flora over the past ten years. The research on maintaining a well-functioning microbiome on our skin when using skin care products is a relatively unexplored area. There are several descriptive studies based on qPCR and NGS of the body's microbiome, but since the composition and richness of the microbiome varies significantly between different skin surfaces, it is not possible to draw general conclusions about the microbiome's influence on a particular product or substance.

Although the skin contains a large number of microorganisms, the presence of an excess of human DNA makes it difficult to apply DNA-based methodology, this is especially true of NGS. Some DNA-based methods are also sensitive to the presence of disruptive substances in the samples, e.g. from skin care products applied to the skin. These challenges mean that optimization work is necessary to get well-functioning and robust methods. Standardized methods for this purpose are often missing.

Summary

Project name

DNA-based methods for skin microbiome

Status

Active

RISE role in project

Project leader

Project start

Duration

2020-2022

Funders

Bo Rydins stiftelse för vetenskaplig forskning

Project members

External press

Supports the UN sustainability goals

3. Good health and well-being
12. Responsible consumption and production
Karin Bjerre

Contact person

Karin Bjerre

Forskare

Read more about Karin

Contact Karin
CAPTCHA This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.

* Mandatory By submitting the form, RISE will process your personal data.

Charlotta Löfström

Contact person

Charlotta Löfström

Senior forskare

Read more about Charlotta

Contact Charlotta
CAPTCHA This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.

* Mandatory By submitting the form, RISE will process your personal data.

Related

Expertise

Microbiological analysis methods

RISE has a broad and deep expertise in microbiological analysis. We develop and validate microbiological methods throughout the analysis chain from sampling and sample preparation to analysis and data processing to efficiently and…
Area

Infection control

Around 700,000 people die every year as a result of infections caused by resistant microorganisms. According to the World Health Organisation, antibiotic resistance poses one of the greatest threats to our health. Rapid developmen…
Area

Chemical and biological analysis

Chemical and biological analyses are essential tools for most types of research, development and production. We provide industry-specific analytical services and expertise across a wide range of fields; for example, industrial pro…
Area

Metrology

Measurements are made all over the world, in all sectors. Usually we measure in order to make decisions or to control something. It can be to decide on certain actions, but it can also be automated decisions in industry control- o…
Service

Molecular biological analyses at genetic level

Using molecular biological analysis methods, we can investigate how different molecules and systems within the cell interact and how these processes are regulated. We can examine differences within the cell system at genome and pr…
Service

DNA sequencing of microorganisms and microflora

With the help of DNA sequencing and bioinformatics, it is possible to identify and characterize microorganisms and microflora in products, processes and in the environment with high precision. We help with all steps in your sequen…